The Regulatory Effect of HOTAIR on the Protein and RNA Levels of ZEB1 and Its Potential Role in the EMT Process

Background: Invasion and metastasis in tumors are considered as two most important reasons for the reduction of treatment efficiency, well an increase mortality rate among patients. According to evidence, HOX transcript antisense RNA (HOTAIR) accounts one main IncRNAs associated with development expansion gastric cancer (GC). Objectives: This research investigates impact HOTAIR suppression on expression translation ZEB1 marker GC. Methods: The knockdown was AGS cell line using small interfering targeting against (si-HOTAIR). proliferation previously evaluated by MTT assay. zinc finger E-box binding homeobox 1 (ZEB1) gene transfected lines quantified compared control samples quantitative real-time polymerase chain reaction (qRT-PCR). enzyme-linked immunosorbent assay (ELISA) also used assess protein. Results: findings revealed that a decrease could cause growth cells (Fold changes = 0/28, P-value < 0.01). levels expression, level directly correlated so led significant decline (P-value 0.05). At protein level, effect observed. Conclusions: Our showed association between levels. Overall, HOTAIR-ZEB1 axis plays vital role epithelial-to-mesenchymal transition (EMT) process human GC represents new therapeutic strategy future treatment.